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¿Están los CLC de Leishmania asociados con la adaptación del parásito a cambios de pH y/o de osmolaridad?
Are the Leishmania CLC Associated with Parasite Adaptation to pH and/or Osmolarity Changes?
DOI:
https://doi.org/10.15446/abc.v21n1Supl.50591Palabras clave:
canal de cloruro CLC, Leishmania, osmolaridad, pH, proteína CLC. (es)CLC chloride channel, CLC protein, Leishmania, Osmolarity, pH (en)
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Leishmania es un protozoario patógeno, que transita durante su ciclo de vida desde el intestino de su vector hasta un compartimiento fagolisosomal al interior de su célula hospedera, el macrófago. Durante este recorrido el parásito está expuesto a ambientes que cambian en temperatura, pH y presumiblemente osmolaridad. Para su supervivencia, Leishmania debe hacer ajustes para adaptarse y la expresión de canales de cloruro ha sido implicada en estos. Basándose en los antecedentes de nuestro grupo de investigación, que registró corrientes de cloruro voltaje dependientes luego de la inyección de mRNA de promastigotes de Leishmania en ovocitos de Xenopues laevis, se postula que éstas son el resultado de la actividad de proteínas CLC. Se mencionan algunas bases moleculares de la adaptación de este párasito con énfasis en transportadores, regulación de calcio, pH y osmolaridad. Con base en datos del grupo se argumenta que la osmolaridad de la vacuola parasitófora es alta. Además se da evidencia de la transcripción en promastigotes de tres genes que codificarían CLC putativos, y se postulan funciones potenciales que llevarían a cabo en los dos estadios del parásito.
Leishmania is a protozoan pathogen that transits during its lifecycle from the gut of the vector to a phagolysosomal compartment within its host cell, the macrophage. During this journey, the parasite is exposed to environments that change in temperature, pH and osmolarity. For their survival, Leishmania must make adjustments to adapt and expression of chloride channels has been involved in these. Based on our research group results: reported voltage-dependent chloride currents after mRNA injection of promastigotes of Leishmania in Xenopus laevis oocytes, it is postulated that these are the result of CLC protein activity. Some molecular bases of adaptation of this parasite are mentioned with emphasis on regulation of calcium, pH and osmolarity. Based on data from our group it is argued that the osmolarity of the parasitophorous vacuole is high. In addition, evidence of the transcription in promastigotes of three genes that encode putative CLC is given and potential functions that take place in the two stages of the parasite are postulated.
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