Publicado

2002-07-01

Improvement of in vitro induction of androgenesis in Phaseolus beans (P. vulgaris L. and P. coccineus L.)

Palabras clave:

anther culture. haploidy, Phaseolus bean, microfluorometry (es)

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Autores/as

  • L Carmenza Muñoz Centro Internacional de Agricultura Tropical, CIAT. Colombia, Cali.
  • Jean Pierre Baudoin Faculte Universitaire des Sciences Agronomiques de Gembloux, Belgica.
Different factors likely tu improve and induttíon in Phaseolus vulgaris L. and P. coccineus L. were tested. Tite highest frequencies of callos índuction were obtained for the P. vulgarls genutypes, \tlth Murashige & Skoog based-medium, suppicmen!ed with kinetin (2 mg/1), 2,4-D (2 mg/1), the vitamins of B5 medium, casein hydrolysate. agarose and a carbon source (l2.5 to 2S g/l of either sucrose or maltose), in the following conditions: 40 antbers in Petri dísbes wtth a diameter Qf 55 mm containing 1O ml of medium, in the dark and at 26°C, with a cold pretreatment of the flower buds of l or 2 days. The analysis of the Calli by microfluorometry revealed a high frequency of haploid cells.

Cómo citar

APA

Muñoz, L. C. y Baudoin, J. P. (2002). Improvement of in vitro induction of androgenesis in Phaseolus beans (P. vulgaris L. and P. coccineus L.). Acta Agronómica, 51(3-4), 81–87. https://revistas.unal.edu.co/index.php/acta_agronomica/article/view/47761

ACM

[1]
Muñoz, L.C. y Baudoin, J.P. 2002. Improvement of in vitro induction of androgenesis in Phaseolus beans (P. vulgaris L. and P. coccineus L.). Acta Agronómica. 51, 3-4 (jul. 2002), 81–87.

ACS

(1)
Muñoz, L. C.; Baudoin, J. P. Improvement of in vitro induction of androgenesis in Phaseolus beans (P. vulgaris L. and P. coccineus L.). Acta Agron. 2002, 51, 81-87.

ABNT

MUÑOZ, L. C.; BAUDOIN, J. P. Improvement of in vitro induction of androgenesis in Phaseolus beans (P. vulgaris L. and P. coccineus L.). Acta Agronómica, [S. l.], v. 51, n. 3-4, p. 81–87, 2002. Disponível em: https://revistas.unal.edu.co/index.php/acta_agronomica/article/view/47761. Acesso em: 28 mar. 2024.

Chicago

Muñoz, L Carmenza, y Jean Pierre Baudoin. 2002. «Improvement of in vitro induction of androgenesis in Phaseolus beans (P. vulgaris L. and P. coccineus L.)». Acta Agronómica 51 (3-4):81-87. https://revistas.unal.edu.co/index.php/acta_agronomica/article/view/47761.

Harvard

Muñoz, L. C. y Baudoin, J. P. (2002) «Improvement of in vitro induction of androgenesis in Phaseolus beans (P. vulgaris L. and P. coccineus L.)», Acta Agronómica, 51(3-4), pp. 81–87. Disponible en: https://revistas.unal.edu.co/index.php/acta_agronomica/article/view/47761 (Accedido: 28 marzo 2024).

IEEE

[1]
L. C. Muñoz y J. P. Baudoin, «Improvement of in vitro induction of androgenesis in Phaseolus beans (P. vulgaris L. and P. coccineus L.)», Acta Agron., vol. 51, n.º 3-4, pp. 81–87, jul. 2002.

MLA

Muñoz, L. C., y J. P. Baudoin. «Improvement of in vitro induction of androgenesis in Phaseolus beans (P. vulgaris L. and P. coccineus L.)». Acta Agronómica, vol. 51, n.º 3-4, julio de 2002, pp. 81-87, https://revistas.unal.edu.co/index.php/acta_agronomica/article/view/47761.

Turabian

Muñoz, L Carmenza, y Jean Pierre Baudoin. «Improvement of in vitro induction of androgenesis in Phaseolus beans (P. vulgaris L. and P. coccineus L.)». Acta Agronómica 51, no. 3-4 (julio 1, 2002): 81–87. Accedido marzo 28, 2024. https://revistas.unal.edu.co/index.php/acta_agronomica/article/view/47761.

Vancouver

1.
Muñoz LC, Baudoin JP. Improvement of in vitro induction of androgenesis in Phaseolus beans (P. vulgaris L. and P. coccineus L.). Acta Agron. [Internet]. 1 de julio de 2002 [citado 28 de marzo de 2024];51(3-4):81-7. Disponible en: https://revistas.unal.edu.co/index.php/acta_agronomica/article/view/47761

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